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Oocytes were treated with either a physiologically relevant (THC1, n = 95, 25 ng/mL 9-THC, 5 ng/mL 11-OH-THC, 50 ng/mL 11-COOH-THC) or a supraphysiologic (THC2, n = 93, 100 ng/mL 9-THC, 50 ng/mL 11-OH-THC, 200 ng/mL 11-COOH-THC) concentration where THC1 is based on the concentration of 9-THC and its metabolites measured in the follicular fluid of IVF patients and THC2 is based on previously reported concentrations in animal studies 23,25,29

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From legal drugs like alcohol and nicotine to powerful opioids such as heroin and fentanyl, these substances hijack the brains reward system, triggering intense cravings, rapid dependence, and challenging withdrawal